The pitfalls of in vivo imaging techniques: evidence for cellular damage caused by synchrotron X-ray computed micro-tomography.
Identifieur interne : 000C32 ( Main/Exploration ); précédent : 000C31; suivant : 000C33The pitfalls of in vivo imaging techniques: evidence for cellular damage caused by synchrotron X-ray computed micro-tomography.
Auteurs : Francesco Petruzzellis [Italie] ; Chiara Pagliarani [Italie] ; Tadeja Savi [Italie, Autriche] ; Adriano Losso [Autriche] ; Silvia Cavalletto [Italie] ; Giuliana Tromba [Italie] ; Christian Dullin [Italie, Allemagne] ; Andreas B R [Autriche] ; Andrea Ganthaler [Autriche] ; Andrea Miotto [Italie] ; Stefan Mayr [Autriche] ; Maciej A. Zwieniecki [États-Unis] ; Andrea Nardini [Italie] ; Francesca Secchi [Italie]Source :
- The New phytologist [ 1469-8137 ] ; 2018.
Descripteurs français
- KwdFr :
- MESH :
- anatomie et histologie : Populus.
- génétique : ARN des plantes.
- métabolisme : Électrolytes.
- méthodes : Imagerie tridimensionnelle.
- Microtomographie aux rayons X, Synchrotrons, Température.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : RNA, Plant.
- chemical , metabolism : Electrolytes.
- anatomy & histology : Populus.
- methods : Imaging, Three-Dimensional.
- Synchrotrons, Temperature, X-Ray Microtomography.
Abstract
Synchrotron X-ray computed micro-tomography (microCT) has emerged as a promising noninvasive technique for in vivo monitoring of xylem function, including embolism build-up under drought and hydraulic recovery following re-irrigation. Yet, the possible harmful effects of ionizing radiation on plant tissues have never been quantified. We specifically investigated the eventual damage suffered by stem living cells of three different species exposed to repeated microCT scans. Stem samples exposed to one, two or three scans were used to measure cell membrane and RNA integrity, and compared to controls never exposed to X-rays. Samples exposed to microCT scans suffered serious alterations to cell membranes, as revealed by marked increase in relative electrolyte leakage, and also underwent severe damage to RNA integrity. The negative effects of X-rays were apparent in all species tested, but the magnitude of damage and the minimum number of scans inducing negative effects were species-specific. Our data show that multiple microCT scans lead to disruption of fundamental cellular functions and processes. Hence, microCT investigation of phenomena that depend on physiological activity of living cells may produce erroneous results and lead to incorrect conclusions.
DOI: 10.1111/nph.15368
PubMed: 30040128
Affiliations:
- Allemagne, Autriche, Italie, États-Unis
- Basse-Saxe, Piémont, Vienne (Autriche)
- Göttingen, Turin, Vienne (Autriche)
Links toward previous steps (curation, corpus...)
Le document en format XML
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Robert-Koch-Straße 40, 37075, Göttingen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Diagnostic and Interventional Radiology, University Medical Center Göttingen, Robert-Koch-Straße 40, 37075, Göttingen</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Göttingen</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Max-Plank-Institute for Experimental Medicine, Hermann-Rein-Straße 3, 37075, Göttingen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Max-Plank-Institute for Experimental Medicine, Hermann-Rein-Straße 3, 37075, Göttingen</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Göttingen</settlement></placeName></affiliation></author><author><name sortKey="B R, Andreas" sort="B R, Andreas" uniqKey="B R A" first="Andreas" last="B R">Andreas B R</name><affiliation wicri:level="1"><nlm:affiliation>Department of Botany, University of Innsbruck, Sternwartestraße 15, 6020, Innsbruck, Austria.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Botany, University of Innsbruck, Sternwartestraße 15, 6020, Innsbruck</wicri:regionArea><wicri:noRegion>Innsbruck</wicri:noRegion></affiliation></author><author><name sortKey="Ganthaler, Andrea" sort="Ganthaler, Andrea" uniqKey="Ganthaler A" first="Andrea" last="Ganthaler">Andrea Ganthaler</name><affiliation wicri:level="1"><nlm:affiliation>Department of Botany, University of Innsbruck, Sternwartestraße 15, 6020, Innsbruck, Austria.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Botany, University of Innsbruck, Sternwartestraße 15, 6020, Innsbruck</wicri:regionArea><wicri:noRegion>Innsbruck</wicri:noRegion></affiliation></author><author><name sortKey="Miotto, Andrea" sort="Miotto, Andrea" uniqKey="Miotto A" 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Giorgieri 10, 34127, Trieste, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze della Vita, Università di Trieste, Via L. Giorgieri 10, 34127, Trieste</wicri:regionArea><wicri:noRegion>Trieste</wicri:noRegion></affiliation></author><author><name sortKey="Mayr, Stefan" sort="Mayr, Stefan" uniqKey="Mayr S" first="Stefan" last="Mayr">Stefan Mayr</name><affiliation wicri:level="1"><nlm:affiliation>Department of Botany, University of Innsbruck, Sternwartestraße 15, 6020, Innsbruck, Austria.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Botany, University of Innsbruck, Sternwartestraße 15, 6020, Innsbruck</wicri:regionArea><wicri:noRegion>Innsbruck</wicri:noRegion></affiliation></author><author><name sortKey="Zwieniecki, Maciej A" sort="Zwieniecki, Maciej A" uniqKey="Zwieniecki M" first="Maciej A" last="Zwieniecki">Maciej A. Zwieniecki</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Sciences, University of California Davis, One Shields Ave, Davis, CA, 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Sciences, University of California Davis, One Shields Ave, Davis, CA, 95616</wicri:regionArea><wicri:noRegion>95616</wicri:noRegion></affiliation></author><author><name sortKey="Nardini, Andrea" sort="Nardini, Andrea" uniqKey="Nardini A" first="Andrea" last="Nardini">Andrea Nardini</name><affiliation wicri:level="1"><nlm:affiliation>Dipartimento di Scienze della Vita, Università di Trieste, Via L. Giorgieri 10, 34127, Trieste, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze della Vita, Università di Trieste, Via L. 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Giorgieri 10, 34127, Trieste, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze della Vita, Università di Trieste, Via L. Giorgieri 10, 34127, Trieste</wicri:regionArea><wicri:noRegion>Trieste</wicri:noRegion></affiliation></author><author><name sortKey="Pagliarani, Chiara" sort="Pagliarani, Chiara" uniqKey="Pagliarani C" first="Chiara" last="Pagliarani">Chiara Pagliarani</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Sustainable Plant Protection, National Research Council, Strada delle Cacce 73, 10135, Torino, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Institute for Sustainable Plant Protection, National Research Council, Strada delle Cacce 73, 10135, Torino</wicri:regionArea><placeName><settlement type="city">Turin</settlement><region type="région" nuts="2">Piémont</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università di Torino, Largo Paolo Braccini 2, 10095, Grugliasco, TO, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università di Torino, Largo Paolo Braccini 2, 10095, Grugliasco, TO</wicri:regionArea><wicri:noRegion>TO</wicri:noRegion></affiliation></author><author><name sortKey="Savi, Tadeja" sort="Savi, Tadeja" uniqKey="Savi T" first="Tadeja" last="Savi">Tadeja Savi</name><affiliation wicri:level="1"><nlm:affiliation>Dipartimento di Scienze della Vita, Università di Trieste, Via L. Giorgieri 10, 34127, Trieste, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze della Vita, Università di Trieste, Via L. Giorgieri 10, 34127, Trieste</wicri:regionArea><wicri:noRegion>Trieste</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>Division of Viticulture and Pomology, Department of Crop Sciences, University of Natural Resources and Life Sciences, Konrad Lorenz Straße 24, A-3430, Tulln, Vienna, Austria.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Division of Viticulture and Pomology, Department of Crop Sciences, University of Natural Resources and Life Sciences, Konrad Lorenz Straße 24, A-3430, Tulln, Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Losso, Adriano" sort="Losso, Adriano" uniqKey="Losso A" first="Adriano" last="Losso">Adriano Losso</name><affiliation wicri:level="1"><nlm:affiliation>Department of Botany, University of Innsbruck, Sternwartestraße 15, 6020, Innsbruck, Austria.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Botany, University of Innsbruck, Sternwartestraße 15, 6020, Innsbruck</wicri:regionArea><wicri:noRegion>Innsbruck</wicri:noRegion></affiliation></author><author><name sortKey="Cavalletto, Silvia" sort="Cavalletto, Silvia" uniqKey="Cavalletto S" first="Silvia" last="Cavalletto">Silvia Cavalletto</name><affiliation wicri:level="1"><nlm:affiliation>Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università di Torino, Largo Paolo Braccini 2, 10095, Grugliasco, TO, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università di Torino, Largo Paolo Braccini 2, 10095, Grugliasco, TO</wicri:regionArea><wicri:noRegion>TO</wicri:noRegion></affiliation></author><author><name sortKey="Tromba, Giuliana" sort="Tromba, Giuliana" uniqKey="Tromba G" first="Giuliana" last="Tromba">Giuliana Tromba</name><affiliation wicri:level="1"><nlm:affiliation>Elettra Sincrotrone Trieste, Area Science Park, 34149, Basovizza, Trieste, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Elettra Sincrotrone Trieste, Area Science Park, 34149, Basovizza, Trieste</wicri:regionArea><wicri:noRegion>Trieste</wicri:noRegion></affiliation></author><author><name sortKey="Dullin, Christian" sort="Dullin, Christian" uniqKey="Dullin C" first="Christian" last="Dullin">Christian Dullin</name><affiliation wicri:level="1"><nlm:affiliation>Elettra Sincrotrone Trieste, Area Science Park, 34149, Basovizza, Trieste, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Elettra Sincrotrone Trieste, Area Science Park, 34149, Basovizza, Trieste</wicri:regionArea><wicri:noRegion>Trieste</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>Institute for Diagnostic and Interventional Radiology, University Medical Center Göttingen, Robert-Koch-Straße 40, 37075, Göttingen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Diagnostic and Interventional Radiology, University Medical Center Göttingen, Robert-Koch-Straße 40, 37075, Göttingen</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Göttingen</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Max-Plank-Institute for Experimental Medicine, Hermann-Rein-Straße 3, 37075, Göttingen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Max-Plank-Institute for Experimental Medicine, Hermann-Rein-Straße 3, 37075, Göttingen</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Göttingen</settlement></placeName></affiliation></author><author><name sortKey="B R, Andreas" sort="B R, Andreas" uniqKey="B R A" first="Andreas" last="B R">Andreas B R</name><affiliation wicri:level="1"><nlm:affiliation>Department of Botany, University of Innsbruck, Sternwartestraße 15, 6020, Innsbruck, Austria.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Botany, University of Innsbruck, Sternwartestraße 15, 6020, Innsbruck</wicri:regionArea><wicri:noRegion>Innsbruck</wicri:noRegion></affiliation></author><author><name sortKey="Ganthaler, Andrea" sort="Ganthaler, Andrea" uniqKey="Ganthaler A" first="Andrea" last="Ganthaler">Andrea Ganthaler</name><affiliation wicri:level="1"><nlm:affiliation>Department of Botany, University of Innsbruck, Sternwartestraße 15, 6020, Innsbruck, Austria.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Botany, University of Innsbruck, Sternwartestraße 15, 6020, Innsbruck</wicri:regionArea><wicri:noRegion>Innsbruck</wicri:noRegion></affiliation></author><author><name sortKey="Miotto, Andrea" sort="Miotto, Andrea" uniqKey="Miotto A" first="Andrea" last="Miotto">Andrea Miotto</name><affiliation wicri:level="1"><nlm:affiliation>Dipartimento di Scienze della Vita, Università di Trieste, Via L. Giorgieri 10, 34127, Trieste, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze della Vita, Università di Trieste, Via L. Giorgieri 10, 34127, Trieste</wicri:regionArea><wicri:noRegion>Trieste</wicri:noRegion></affiliation></author><author><name sortKey="Mayr, Stefan" sort="Mayr, Stefan" uniqKey="Mayr S" first="Stefan" last="Mayr">Stefan Mayr</name><affiliation wicri:level="1"><nlm:affiliation>Department of Botany, University of Innsbruck, Sternwartestraße 15, 6020, Innsbruck, Austria.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Botany, University of Innsbruck, Sternwartestraße 15, 6020, Innsbruck</wicri:regionArea><wicri:noRegion>Innsbruck</wicri:noRegion></affiliation></author><author><name sortKey="Zwieniecki, Maciej A" sort="Zwieniecki, Maciej A" uniqKey="Zwieniecki M" first="Maciej A" last="Zwieniecki">Maciej A. Zwieniecki</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Sciences, University of California Davis, One Shields Ave, Davis, CA, 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Sciences, University of California Davis, One Shields Ave, Davis, CA, 95616</wicri:regionArea><wicri:noRegion>95616</wicri:noRegion></affiliation></author><author><name sortKey="Nardini, Andrea" sort="Nardini, Andrea" uniqKey="Nardini A" first="Andrea" last="Nardini">Andrea Nardini</name><affiliation wicri:level="1"><nlm:affiliation>Dipartimento di Scienze della Vita, Università di Trieste, Via L. Giorgieri 10, 34127, Trieste, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze della Vita, Università di Trieste, Via L. Giorgieri 10, 34127, Trieste</wicri:regionArea><wicri:noRegion>Trieste</wicri:noRegion></affiliation></author><author><name sortKey="Secchi, Francesca" sort="Secchi, Francesca" uniqKey="Secchi F" first="Francesca" last="Secchi">Francesca Secchi</name><affiliation wicri:level="1"><nlm:affiliation>Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università di Torino, Largo Paolo Braccini 2, 10095, Grugliasco, TO, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università di Torino, Largo Paolo Braccini 2, 10095, Grugliasco, TO</wicri:regionArea><wicri:noRegion>TO</wicri:noRegion></affiliation></author></analytic><series><title level="j">The New phytologist</title><idno type="eISSN">1469-8137</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Electrolytes (metabolism)</term><term>Imaging, Three-Dimensional (methods)</term><term>Populus (anatomy & histology)</term><term>RNA, Plant (genetics)</term><term>Synchrotrons (MeSH)</term><term>Temperature (MeSH)</term><term>X-Ray Microtomography (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN des plantes (génétique)</term><term>Imagerie tridimensionnelle (méthodes)</term><term>Microtomographie aux rayons X (MeSH)</term><term>Populus (anatomie et histologie)</term><term>Synchrotrons (MeSH)</term><term>Température (MeSH)</term><term>Électrolytes (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Electrolytes</term></keywords><keywords scheme="MESH" qualifier="anatomie et histologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN des plantes</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Imaging, Three-Dimensional</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Électrolytes</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Imagerie tridimensionnelle</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Synchrotrons</term><term>Temperature</term><term>X-Ray Microtomography</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Microtomographie aux rayons X</term><term>Synchrotrons</term><term>Température</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Synchrotron X-ray computed micro-tomography (microCT) has emerged as a promising noninvasive technique for in vivo monitoring of xylem function, including embolism build-up under drought and hydraulic recovery following re-irrigation. Yet, the possible harmful effects of ionizing radiation on plant tissues have never been quantified. We specifically investigated the eventual damage suffered by stem living cells of three different species exposed to repeated microCT scans. Stem samples exposed to one, two or three scans were used to measure cell membrane and RNA integrity, and compared to controls never exposed to X-rays. Samples exposed to microCT scans suffered serious alterations to cell membranes, as revealed by marked increase in relative electrolyte leakage, and also underwent severe damage to RNA integrity. The negative effects of X-rays were apparent in all species tested, but the magnitude of damage and the minimum number of scans inducing negative effects were species-specific. Our data show that multiple microCT scans lead to disruption of fundamental cellular functions and processes. Hence, microCT investigation of phenomena that depend on physiological activity of living cells may produce erroneous results and lead to incorrect conclusions.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30040128</PMID><DateCompleted><Year>2019</Year><Month>10</Month><Day>01</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1469-8137</ISSN><JournalIssue CitedMedium="Internet"><Volume>220</Volume><Issue>1</Issue><PubDate><Year>2018</Year><Month>10</Month></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>The pitfalls of in vivo imaging techniques: evidence for cellular damage caused by synchrotron X-ray computed micro-tomography.</ArticleTitle><Pagination><MedlinePgn>104-110</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/nph.15368</ELocationID><Abstract><AbstractText>Synchrotron X-ray computed micro-tomography (microCT) has emerged as a promising noninvasive technique for in vivo monitoring of xylem function, including embolism build-up under drought and hydraulic recovery following re-irrigation. Yet, the possible harmful effects of ionizing radiation on plant tissues have never been quantified. We specifically investigated the eventual damage suffered by stem living cells of three different species exposed to repeated microCT scans. Stem samples exposed to one, two or three scans were used to measure cell membrane and RNA integrity, and compared to controls never exposed to X-rays. Samples exposed to microCT scans suffered serious alterations to cell membranes, as revealed by marked increase in relative electrolyte leakage, and also underwent severe damage to RNA integrity. The negative effects of X-rays were apparent in all species tested, but the magnitude of damage and the minimum number of scans inducing negative effects were species-specific. Our data show that multiple microCT scans lead to disruption of fundamental cellular functions and processes. Hence, microCT investigation of phenomena that depend on physiological activity of living cells may produce erroneous results and lead to incorrect conclusions.</AbstractText><CopyrightInformation>© 2018 The Authors. New Phytologist © 2018 New Phytologist Trust.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Petruzzellis</LastName><ForeName>Francesco</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Dipartimento di Scienze della Vita, Università di Trieste, Via L. Giorgieri 10, 34127, Trieste, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pagliarani</LastName><ForeName>Chiara</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Institute for Sustainable Plant Protection, National Research Council, Strada delle Cacce 73, 10135, Torino, Italy.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università di Torino, Largo Paolo Braccini 2, 10095, Grugliasco, TO, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Savi</LastName><ForeName>Tadeja</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Dipartimento di Scienze della Vita, Università di Trieste, Via L. Giorgieri 10, 34127, Trieste, Italy.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Division of Viticulture and Pomology, Department of Crop Sciences, University of Natural Resources and Life Sciences, Konrad Lorenz Straße 24, A-3430, Tulln, Vienna, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Losso</LastName><ForeName>Adriano</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Botany, University of Innsbruck, Sternwartestraße 15, 6020, Innsbruck, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cavalletto</LastName><ForeName>Silvia</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università di Torino, Largo Paolo Braccini 2, 10095, Grugliasco, TO, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tromba</LastName><ForeName>Giuliana</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Elettra Sincrotrone Trieste, Area Science Park, 34149, Basovizza, Trieste, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dullin</LastName><ForeName>Christian</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Elettra Sincrotrone Trieste, Area Science Park, 34149, Basovizza, Trieste, Italy.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institute for Diagnostic and Interventional Radiology, University Medical Center Göttingen, Robert-Koch-Straße 40, 37075, Göttingen, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Max-Plank-Institute for Experimental Medicine, Hermann-Rein-Straße 3, 37075, Göttingen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bär</LastName><ForeName>Andreas</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Botany, University of Innsbruck, Sternwartestraße 15, 6020, Innsbruck, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ganthaler</LastName><ForeName>Andrea</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Botany, University of Innsbruck, Sternwartestraße 15, 6020, Innsbruck, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Miotto</LastName><ForeName>Andrea</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Dipartimento di Scienze della Vita, Università di Trieste, Via L. Giorgieri 10, 34127, Trieste, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mayr</LastName><ForeName>Stefan</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Botany, University of Innsbruck, Sternwartestraße 15, 6020, Innsbruck, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zwieniecki</LastName><ForeName>Maciej A</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Department of Plant Sciences, University of California Davis, One Shields Ave, Davis, CA, 95616, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nardini</LastName><ForeName>Andrea</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Dipartimento di Scienze della Vita, Università di Trieste, Via L. Giorgieri 10, 34127, Trieste, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Secchi</LastName><ForeName>Francesca</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università di Torino, Largo Paolo Braccini 2, 10095, Grugliasco, TO, Italy.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>07</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>New Phytol</MedlineTA><NlmUniqueID>9882884</NlmUniqueID><ISSNLinking>0028-646X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004573">Electrolytes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004573" MajorTopicYN="N">Electrolytes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021621" MajorTopicYN="N">Imaging, Three-Dimensional</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018749" MajorTopicYN="N">RNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017356" MajorTopicYN="Y">Synchrotrons</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055114" MajorTopicYN="Y">X-Ray Microtomography</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">RNA
</Keyword><Keyword MajorTopicYN="Y">X-ray damage</Keyword><Keyword MajorTopicYN="Y">cell membrane</Keyword><Keyword MajorTopicYN="Y">hydraulic recovery</Keyword><Keyword MajorTopicYN="Y">microCT</Keyword><Keyword MajorTopicYN="Y">xylem embolism</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>06</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>07</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>7</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>10</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>7</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30040128</ArticleId><ArticleId IdType="doi">10.1111/nph.15368</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>Autriche</li><li>Italie</li><li>États-Unis</li></country><region><li>Basse-Saxe</li><li>Piémont</li><li>Vienne (Autriche)</li></region><settlement><li>Göttingen</li><li>Turin</li><li>Vienne (Autriche)</li></settlement></list><tree><country name="Italie"><noRegion><name sortKey="Petruzzellis, Francesco" sort="Petruzzellis, Francesco" uniqKey="Petruzzellis F" first="Francesco" last="Petruzzellis">Francesco Petruzzellis</name></noRegion><name sortKey="Cavalletto, Silvia" sort="Cavalletto, Silvia" uniqKey="Cavalletto S" first="Silvia" last="Cavalletto">Silvia Cavalletto</name><name sortKey="Dullin, Christian" sort="Dullin, Christian" uniqKey="Dullin C" first="Christian" last="Dullin">Christian Dullin</name><name sortKey="Miotto, Andrea" sort="Miotto, Andrea" uniqKey="Miotto A" first="Andrea" last="Miotto">Andrea Miotto</name><name sortKey="Nardini, Andrea" sort="Nardini, Andrea" uniqKey="Nardini A" first="Andrea" last="Nardini">Andrea Nardini</name><name sortKey="Pagliarani, Chiara" sort="Pagliarani, Chiara" uniqKey="Pagliarani C" first="Chiara" last="Pagliarani">Chiara Pagliarani</name><name sortKey="Pagliarani, Chiara" sort="Pagliarani, Chiara" uniqKey="Pagliarani C" first="Chiara" last="Pagliarani">Chiara Pagliarani</name><name sortKey="Savi, Tadeja" sort="Savi, Tadeja" uniqKey="Savi T" first="Tadeja" last="Savi">Tadeja Savi</name><name sortKey="Secchi, Francesca" sort="Secchi, Francesca" uniqKey="Secchi F" first="Francesca" last="Secchi">Francesca Secchi</name><name sortKey="Tromba, Giuliana" sort="Tromba, Giuliana" uniqKey="Tromba G" first="Giuliana" last="Tromba">Giuliana Tromba</name></country><country name="Autriche"><region name="Vienne (Autriche)"><name sortKey="Savi, Tadeja" sort="Savi, Tadeja" uniqKey="Savi T" first="Tadeja" last="Savi">Tadeja Savi</name></region><name sortKey="B R, Andreas" sort="B R, Andreas" uniqKey="B R A" first="Andreas" last="B R">Andreas B R</name><name sortKey="Ganthaler, Andrea" sort="Ganthaler, Andrea" uniqKey="Ganthaler A" first="Andrea" last="Ganthaler">Andrea Ganthaler</name><name sortKey="Losso, Adriano" sort="Losso, Adriano" uniqKey="Losso A" first="Adriano" last="Losso">Adriano Losso</name><name sortKey="Mayr, Stefan" sort="Mayr, Stefan" uniqKey="Mayr S" first="Stefan" last="Mayr">Stefan Mayr</name></country><country name="Allemagne"><region name="Basse-Saxe"><name sortKey="Dullin, Christian" sort="Dullin, Christian" uniqKey="Dullin C" first="Christian" last="Dullin">Christian Dullin</name></region><name sortKey="Dullin, Christian" sort="Dullin, Christian" uniqKey="Dullin C" first="Christian" last="Dullin">Christian Dullin</name></country><country name="États-Unis"><noRegion><name sortKey="Zwieniecki, Maciej A" sort="Zwieniecki, Maciej A" uniqKey="Zwieniecki M" first="Maciej A" last="Zwieniecki">Maciej A. Zwieniecki</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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